Monoclonal Antibody Against the Common p Subunit ( Pc ) of the Human Interleukin - 3 ( IL - 3 ) , IL - 5 , and Granulocyte - Macrophage Colony - Stimulating Factor Receptors
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چکیده
High-affinity receptors for human granulocyte macrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3). and IL-5 are composed of two distinct subunits, a and p. Each receptor has its own ligand-specific a subunit, and the three receptors share the common p subunit, BE. Using a transfectant of NIH3T3 cells expressing the high-affinity human GM-CSF receptor, monoclonal antibodies (MoAbs) against pc were generated. These MoAbs specifically bound to cells bearing pc and immunoprecipitated the pc protein of 120 Kd. Using these MoAbs, expression of pc was examined. It is known that IL-1 augments the proliferative response of a
منابع مشابه
RAPID COMMUNICATION Hematopoiesis in Mice Lacking the Entire Granulocyte-Macrophage Colony-Stimulating Factor/Interleukin-3/Interleukin-5 Functions
Interleukin-3 (IL-3). granulocyte-macrophage colony-stimulating factor (GM-CSF), and IL-5 are major hematopoietic cytokines produced by activated T cells and exhibit similar biologic activities by signaling through a common receptor subunit (pc). Mice lacking p c show a pulmonary alveolar proteinosis-like disease and reduced numbers of peripheral eosinophils, which are explained by the lack of ...
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